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WIREs RNA
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Advances in the characterization of RNA‐binding proteins

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From transcription, to transport, storage, and translation, RNA depends on association with different RNA‐binding proteins (RBPs). Methods based on next‐generation sequencing and protein mass‐spectrometry have started to unveil genome‐wide interactions of RBPs but many aspects still remain out of sight. How many of the binding sites identified in high‐throughput screenings are functional? A number of computational methods have been developed to analyze experimental data and to obtain insights into the specificity of protein–RNA interactions. How can theoretical models be exploited to identify RBPs? In addition to oligomeric complexes, protein and RNA molecules can associate into granular assemblies whose physical properties are still poorly understood. What protein features promote granule formation and what effects do these assemblies have on cell function? Here, we describe the newest in silico, in vitro, and in vivo advances in the field of protein–RNA interactions. We also present the challenges that experimental and computational approaches will have to face in future studies. WIREs RNA 2016, 7:793–810. doi: 10.1002/wrna.1378 This article is categorized under: RNA Interactions with Proteins and Other Molecules > Protein–RNA Recognition RNA Interactions with Proteins and Other Molecules > RNA–Protein Complexes RNA in Disease and Development > RNA in Disease
RNA‐binding proteins (RBPs) and RNA life. RNA birth is regulated by RBPs (1) that are responsible for maturation (2) and modification (3). RBPs protect (4) and transport (5) RNA around the cell to specific sites (6). Interactions are regulated through a diverse set of binding sites that allows the formation of dynamic complexes sustained by reversible contacts and involving multiple partners (7, 8). When RNA is not required, it can be stored for future needs (7) or degraded (8). The last process of the RNA life cycle is the release of nucleotides that will be employed to build new RNA molecules. When RBPs are impaired (e.g., protein mutation, concentration deregulation, etc.), half‐life, arrangement and location of RNA are affected (9).
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Features of yeast proteins forming ribonucleoprotein assemblies. Using physicochemical properties, the multicleverMachine approach discriminates P‐bodies and stress granules from other globular proteins. The datasets employed in this analysis comprise 52 P‐body and 62 stress granule proteins, previously reported in experimental works, as well as five random sets of 100 globular proteins from Astral SCOPe 2.05 (<40% sequence identity). Three specific properties are reported: (a) nucleic acid binding, (b) hydrophobicity, and (c) disorder propensity. For each feature, enrichment or depletion of a set is indicated with a specific color: green indicates that proteins contained in P‐bodies or stress granules are enriched with respect to proteome subsamples; red means depletion with respect to the proteome subsamples; yellow indicates no significant differences between the sets. As observed in previous experimental works, proteins found in P‐bodies and stress granules are more structurally disordered and prone to bind nucleic acids as well as less hydrophobic (p‐values <10−5; Fisher's exact test). Sets and results can be accessed at http://www.tartaglialab.com/cs_multi/confirm/1207/c51ef6cff3/ where additional statistical analyses are also reported.
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Methods to predict RNA binding sites. We calculated performances of BindN, BindN+, Pprint, RNAproB, RNABindR+, and catRAPID signature on a set of proteins whose RNA‐binding sites have been validated through X‐ray and NMR techniques. As in a recent work, three protein classes ‘fold,’ ‘family,’ and ‘superfamily’ were retrieved from SCOP. Performances were estimated using the formula (sensitivity + specificity)/2 on 90 folds, 126 families, and 100 superfamilies (details at http://service.tartaglialab.com/static_files/shared/documentation_signature.html).
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Schematic representation of experimental methods for the identification of protein–RNA interactions. Protein‐centric methods. (a) In vivo approaches include native purification protocols (RNA Immunoprecipitation, RIP) and denaturing protocols (Cross‐linking and Immunoprecipitation, CLIP). In the fist case, RNAs bound to a specific protein are immunoprecipitated from cell lysate in native conditions by using a protein‐specific antibody and, after wash and protein removal by proteinase K treatment, RNAs are reverse transcribed and identified through RNA sequencing. In the second case, cells are UV cross‐linked to ‘freeze’ protein–RNA complexes. RNA is digested to obtain fragments of a defined size and the obtained complexes are immunoprecipitated and resolved on an SDS‐PAGE. After isolation from membrane and proteinase K digestion, the RNA fragments are reverse transcribed and sequenced. In red and yellow are represented simulated enrichment values of a target and nontarget RNA respectively (lower part of the panel). (b) As an example of in vitro approaches, the schematic workflow of RNA compete is represented. RNA libraries are generated by in vitro transcription. Transcripts are incubated with the protein of interest immobilized on an affinity matrix (e.g., streptavidin‐biotin tag system) and the bound fragments are then fluorescently labeled and detected by hybridization on a microarray platform. RNA‐centric methods. (c) In vivo approaches for the identification of proteins bound to an RNA of interest often derive from methods used for the identification of genomic DNA loci targeted by noncoding RNAs. Cells are cross‐linked and lysed. Chromatin is sheared and protein–RNA–DNA complexes are pull‐down by using biotinylated oligos complementary to the sequence of the RNA. After RNA digestion, proteins can be identified by western blot or mass spectrometry analysis. (d) In vitro approaches commonly exploit the use of RNA tags to immobilize the RNA of interest onto an affinity matrix. Upon incubation with cell lysate, proteins bind to the immobilized RNA. After washes, the protein–RNA complexes are eluted from the matrix and proteins are characterized by western blot or mass spectrometry.
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RNA Interactions with Proteins and Other Molecules > Protein–RNA Recognition
RNA Interactions with Proteins and Other Molecules > RNA–Protein Complexes
RNA in Disease and Development > RNA in Disease

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