Home
This Title All WIREs
WIREs RSS Feed
How to cite this WIREs title:
WIREs RNA
Impact Factor: 6.154

No‐go decay: a quality control mechanism for RNA in translation

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

Eukaryotic cells have evolved multiple quality control mechanisms that recognize and eliminate defective mRNA during the process of translation. One mechanism, referred to as No‐go decay (NGD), targets mRNAs with elongation stalls for degradation initiated by endonucleolytic cleavage in the vicinity of the stalled ribosome. NGD is promoted by the evolutionarily conserved Dom34 and Hbs1 proteins, which are related to the translation termination factors eRF1 and eRF3, respectively. NGD is likely to occur by Dom34/Hbs1 interacting with the A site in the ribosome leading to release of the peptide or peptidyl‐tRNA. The process of NGD and/or the function of Dom34/Hbs1 appear to be important in several different biological contexts. Copyright © 2010 John Wiley & Sons, Ltd.

Figure 1.

Working model for no‐go decay. During translation elongation, the ribosome can be paused for a variety of reasons. If the A site is empty during the prolonged elongation stall, it allows the Dom34/Hbs1 complex to bind to the A site of the stalled ribosome leading to peptidyl‐tRNA hydrolysis and release of the peptide, or release of the peptidyl‐tRNA. At this stage, three events could take place: (1) endonucleolytic cleavage of the mRNA in the vicinity of the stalled ribosome, (2) release of the stalled ribosome, and (3) degradation of the nascent peptide or the peptidyl‐tRNA via the ubiquitin (Ub)–proteasome system. Note that mRNA cleavage could possibly occur before the peptidyl‐tRNA hydrolysis or the release of the peptidyl‐tRNA. In any case, the mRNA fragments would be exonucleolytically digested by the exosome and Xrn1. The released ribosome might undergo disassembly and degradation. The released tRNA might be recycled.

[ Normal View | Magnified View ]

Related Articles

Bacillus subtilis mRNA decay: new parts in the toolkit
Exonucleases and endonucleases involved in polyadenylation‐ assisted RNA decay
Mechanisms of deadenylation‐dependent decay
Mechanisms of endonuclease‐mediated mRNA decay
Networks controlling mRNA decay in the immune system
RNA decay modulates gene expression and controls its fidelity
The role of AUF1 in regulated mRNA decay
The role of KSRP in mRNA decay and microRNA precursor maturation
The roles of TTP and BRF proteins in regulated mRNA decay
Viruses and the cellular RNA decay machinery

Browse by Topic

RNA Turnover and Surveillance > Turnover/Surveillance Mechanisms
RNA Turnover and Surveillance > Regulation of RNA Stability
blog comments powered by Disqus

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts

Twitter: molecular Follow us on Twitter

    Get all the FREE Current Protocols e-Alerts, Webinar News, and Troubleshooting Tips you’ll need for your bench work with ONE EASY CLICK -...
    Preclinical safety studies of a new potential drug for Hunter syndrome demonstrate its safety and efficiency http://t.co/bhc1yNQ90A
    Wiley to Provide Emergency Access to Biomedical Literature to Aid Ebola Outbreak Relief Efforts http://t.co/af3rqGoP0Y