Home
This Title All WIREs
WIREs RSS Feed
How to cite this WIREs title:
WIREs Syst Biol Med
Impact Factor: 3.542

Isotope labeling experiments in metabolomics and fluxomics

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

Abstract Metabolomics, the study of all the small molecules in and outside a cell and fluxomics, comprising all conversion rates in a cell, are increasingly used in fundamental and applied sciences to unravel structures and activities of cellular networks and their regulation, to investigate mechanisms of diseases and toxicity, and to improve producing strains among other applications. For both fluxomics and metabolomics the application of isotopes became almost indispensable. Their use in these techniques is discussed, focusing primarily on studies applying stable isotopes and using mass spectrometry. This includes the underlying principles, experimental and computational methods used, and examples of application. WIREs Syst Biol Med 2012 doi: 10.1002/wsbm.1167 This article is categorized under: Analytical and Computational Methods > Analytical Methods Models of Systems Properties and Processes > Cellular Models Laboratory Methods and Technologies > Metabolomics

This WIREs title offers downloadable PowerPoint presentations of figures for non-profit, educational use, provided the content is not modified and full credit is given to the author and publication.

Download a PowerPoint presentation of all images


The eukaryotic cell including the flow of information from the genome to the metabolome (white arrows) and their interaction in terms of regulation (gray arrows). The hierarchy in the network is displayed by the prefixed numbers.

[ Normal View | Magnified View ]

Carbon isotopomers and labeling transfer in metabolism. (a) Isotopomers of a molecule with three carbon atoms, e.g., pyruvate or alanine. Eight carbon isotopomers and four mass isotopomers are depicted. (b) Principles of carbon transfer starting with [1 −13C] glucose. Degradation of three molecules of glucose via glycolysis yields three labeled and three unlabeled C3 molecules each. Metabolism via the PPP with the loss of all the labeling information resulting in five unlabeled pyruvate molecules. Using the ratio of unlabeled and labeled pyruvate it is possible to calculate the participation of each pathway to the degradation of glucose.

[ Normal View | Magnified View ]

Experimental procedure in metabolomics and fluxomics and possible applications for isotopes.

[ Normal View | Magnified View ]

Connection of metabolome and fluxome measurements exemplified with a simple consecutive reaction with metabolites M1, M2, and M3 and rates r1 and r2. Michaelis–Menten‐type kinetics is assumed. The two horizontal lines, dashed and dotted, indicate two stationary fluxes, r1 = r2. Vertical dashed lines indicate measured metabolite concentrations, CM1. Cases a/b and a′/b′ indicate two different flux states with corresponding rates. Cases a/c and a′/c′ indicate situations with lower concentration of CM2 and higher expression of the enzyme catalyzing reaction 2.

[ Normal View | Magnified View ]

Related Articles

The application of flux balance analysis in systems biology
Genome‐scale metabolic networks

Browse by Topic

Analytical and Computational Methods > Analytical Methods
Laboratory Methods and Technologies > RNA Methods
Models of Systems Properties and Processes > Cellular Models

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts