Home
This Title All WIREs
WIREs RSS Feed
How to cite this WIREs title:
WIREs Syst Biol Med
Impact Factor: 2.385

Cardiac lineage selection: integrating biological complexity into computational models

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

The emergence of techniques to study developmental processes using systems biology approaches offers exciting possibilities for the develpmental biologist. In particular cardiac lineage selection may be particularly amenable to these types of studies since the heart is the first fully functional organ to form in vertebrates. However there are many technical obstacles that need to be overcome for these studies to proceed. Here we present a brief overview of cardiomyocyte lineage deterimination and discuss how different aspects of this process either benefit from or present unique challenges for the development of systems biology approaches. Copyright © 2009 John Wiley & Sons, Inc.

This article is categorized under:

  • Developmental Biology > Developmental Processes in Health and Disease

This WIREs title offers downloadable PowerPoint presentations of figures for non-profit, educational use, provided the content is not modified and full credit is given to the author and publication.

Download a PowerPoint presentation of all images


Figure 1.

A graphical representation of the movement of heart precursors in an amphibian embryo from the beginning of gastrulation to the early tailbud at which point heart cells have migrated to their final destination in the embryo. The red spot marks the approximate location of heart precursors at that stage and the arrow represents the direction of their migration. (Redrawn after Copenhaver in Refs3, 4).

[ Normal View | Magnified View ]
Figure 2.

Schematic overview of cardiac lineage selection. All cells in the embryo start out as undifferentiated epiblast. Through the process of gastrulation, the three germ layers, ectoderm, mesoderm, and endoderm, form. Mesoderm and endoderm initially exit from the streak as a mixed mesendodermal population marked by expression of the transcription factor T/brachyury but quickly sorts out into morphologically and molecularly distinct mesoderm and endoderm. Mesodermal cells then take on specific regional identities under the influence of environmental cues. At this stage, cells destined to form the primary heart tube express the transcription factors Nkx2.5 and Tbx5. Finally, these cells go on to form beating cardiomyocytes that express myosin heavy chain α and other contractile proteins.

[ Normal View | Magnified View ]
Figure 3.

Wire diagram of the major molecular interactions that are key to the establishment and early functioning of the dorsal organizing centers. Essentially, canonical Wnt/β‐Catenin (β‐Cat) signaling through disheveled (dsh) disrupts the β‐Catenin destruction complex [consisting of adenomatous polyposis coli (APC), Axin and glycogen, synthase kinase 3 (gsk3β)], which allows for the nuclear localization of β‐Catenin and establishes the expression of dorsal molecular markers such as twins, siamois, and goosecoid and establishes a domain of nodal signaling. Nodal and other members of the transforming growth factor β (TGFβ) family of secreted factors, signaling through receptor serine/threonine kinases (RS/Tks) or activin receptors (ActIIR, ALK4) establish markers for the dorsal mesoderm and endoderm including brachyury (bra) and other markers for the dorsal mesoderm and endoderm such as cerberus, lim‐1 (lim), and dikkopf‐1 (dkk1). Explanation of symbols used can be found in Table 1.

[ Normal View | Magnified View ]
Figure 4.

Wire diagram of the major signaling networks that induce the expression of early mesendodermal markers. At this stage, the FGF signaling pathway interacts closely with members of the TGFβ family of secreted factors signaling through receptor serine/threonine kinases (RS/Tks) or activin receptors (ActIIR, ALK4) to establish and maintain the expression of early markers for the mesoderm and endoderm and also establishes chordin (chd), dhand, ehand, and sonic hedgehog (shh) as well as endodermal markers such as sox17 and foxA2 (other abbreviations are the same as in Figure 3). Explanation of symbols used can be found in Table 1.

[ Normal View | Magnified View ]
Figure 5.

Waddington's Epigenetic Landscape. While clearly no longer an accurate representation of the emerging field of epigenetics, still a useful visual tool to describe the phenomenon by which developmental fates become progressively limited. The downward slope represents developmental time. As the ball (cell) rolls down the slope (differentiates), its path will follow one of a few possible paths. At each node, the developmental potential of the cell becomes progressively more limited until it reaches a point at which it has only one possible fate.58 In the context of this review, each fork in the developmental path likely represents a local change in the transcriptional state of the differentiating cell.

[ Normal View | Magnified View ]
Figure 6.

A partial, non‐comprehensive wire diagram summarizing some of the factors known to play key roles in cardiomyocyte lineage selection and their relative positions within the cell. The main signaling feature at this phase of development is mediated by antagonists of the canonical Wnt/β‐Catenin signaling pathway. Normally, signaling through the β‐Catenin pathway inhibits the expression of genes such as Hex, Naked (nkd) and nemo‐like kinase (nlk); however, inhibition of this pathway by Wnt11 and Dkk‐1 allows for the transcription of these factors. In addition, nodal signaling patterns both endodermal (cerberus, mix, FoxH1) and mesodermal (Nkx2.5 and Tbx5). For the sake of simplicity, both of these are shown on the same diagram. In addition, other signaling pathways that are thought to play roles in the maintenance and proliferation of cardiac phenotypes at these stages (BMP and FGF) are not shown in this diagram (other abbreviations same as in Figures 3 and 4).

[ Normal View | Magnified View ]

Related Articles

Signals controlling neural crest contributions to the heart
Multiscale modeling for biologists
Emerging clinical applications in cardiovascular pharmacogenomics
Image‐based models of cardiac structure in health and disease
Signaling pathways in early cardiac development
Integrative Bioinformatics

Browse by Topic

Developmental Biology > Developmental Processes in Health and Disease

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts

In the Spotlight

Jens Nielsen

Jens Nielsen
is a Professor in the Department of Biology and Biological Engineering at Chalmers University of Technology in Göteborg, Sweden. His research focus is on systems biology of metabolism. The yeast Saccharomyces cerevisiae is the lab’s key organism for experimental research, but they also work with Aspergilli oryzae.

Learn More