A perfect RNA force field and convergent sampling would generate a free energy landscape to reliably predict structure and dynamics.

Recent structural studies provide an atomic view of substrate recognition and cleavage by the VS ribozyme.

An overview of techniques used to interrogate in vitro, in vivo, and genome‐wide techniques used to study RNA‐protein interactions.(A‐B) In vitro techniques. (A) Electrophoretic mobility shift assays (EMSAs). (B) Systematic evolution of ligands by exponential enrichment (SELEX). (C‐E) Target‐specific in vivo techniques. (C) RNA immunoprecipitation (RIP). RIP can be followed by RT‐qPCR (RIP‐qPCR), microarray (RIP‐chip), or RNA sequencing (RIP‐seq). (D) Crosslinking followed by Immunoprecipitation (CLIP). CLIP can be followed by RT‐qPCR (CLIP‐qPCR), microarray (CLIP‐chip), or RNA sequencing (CLIP‐seq). (E) Photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR‐CLIP). (F‐G) Genome‐wide techniques. (F) Global photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (gPAR‐CLIP). (G) Protein interaction profile sequencing (PIP‐seq).

tRFs derive from tRNAs and constitute a novel and heterogeneous class of small non‐coding RNAs involved in normal cell biology and in cancer, neurodegenerative disorders, and viral infections.

mRNA decay provides agility to the Cryptococcus neoformans transcriptome during both the response to and recovery from stress.13

RNA binding proteins influence the expression of cell cycle regulators, which then in turn affects the balance of cell cycle progression and V(D)J recombination in developing lymphocytes. In this review, we discuss how RNA binding protein (RBP) regulates the cell cycle and other pathways in developing lymphocytes.