Home
This Title All WIREs
WIREs RSS Feed
How to cite this WIREs title:
WIREs Nanomed Nanobiotechnol
Impact Factor: 7.689

Recent advances of using polyhydroxyalkanoate‐based nanovehicles as therapeutic delivery carriers

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

Polyhydroxyalkanoates (PHAs) are a class of diverse biodegradable polyesters that can be produced either by natural bioconversion process or by chemical synthesis via the ring‐opening polymerization of β‐lactones. Because of the excellent biodegradability and biocompatibility, the development of PHA‐based nanovehicles as therapeutic delivery carriers, including nanoparticles, micelles, liposomes, and vesicles, has received considerable attention in recent years, and these sophisticated materials have demonstrated significant impact on the drug bioavailability, better encapsulation, and less toxic properties of biodegradable polymers. In this review, the most recent advances of using PHA‐based nanovehicles as therapeutic delivery carriers are summarized with respect to different material types including intrinsic bulk PHA and functionalized PHA through biological and chemical approaches. The bio‐significance of using different carriers in the controlled and targeted delivery of hydrophobic drugs, proteins, vaccines, nucleic acids (DNA and siRNA), and biological macromolecules in cancer therapy is also discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1429. doi: 10.1002/wnan.1429 This article is categorized under: Implantable Materials and Surgical Technologies > Nanomaterials and Implants Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement
Different synthentic approaches of polyhydroxyalkanoate (PHA). (a) Biosynthetic pathway of producing natural isotactic poly(3‐hydroxybutyrate) (PHB) and (b) chemical synthesis of PHA with various microstructures, illustrated in the case of PHB.
[ Normal View | Magnified View ]
PDMAEMA‐PHB‐PDMAEMA triblock copolymers are denoted as DHD, and the numbers in brackets show the indicative molecular weight of the respective block in 100 g/mol. The effect of temperature (a) and pH (b) on the drug release profile of PDMAEMA‐PHB‐PDMAEMA micelles. Confocal images of HeLa cells uptake by incubating with (c) Pluronic F127 micelles and (d) PDMAEMA‐PHB‐PDMAEMA, DHD(21–126) micelles. The scale bar denotes 20 mm. (Reprinted with permission from Ref . Copyright 2013 Royal Society of Chemistry). (e) Schematic illustration of the DHD‐condensed plasmid polyplexes in gene delivery. (Reprinted with permission from Ref . Copyright 2013 Wiley‐VCH)
[ Normal View | Magnified View ]
PNIPAAm‐PHB‐PNIPAAm triblock copolymer and its stimuli responsive properties. (a) Synthesis of PNIPAAm‐PHB‐PNIPAAm triblock copolymer using PHB‐diBr as macroinitiator in ATRP and (b) thermoresponsive demonstration of PNIPAAm‐PHB‐PNIPAAm triblock copolymers. (Reprinted with permission from Ref . Copyright 2009 American Chemical Society)
[ Normal View | Magnified View ]
PEG‐PHB‐PEG triblock copolymer based self‐assemblies for drug delivery application. (a) Preparation of PEG‐PHB‐PEG triblock copolymers and (b) schematic of PEG‐PHB‐PEG triblock copolymer and α‐CD. (c) Optical photographs of the PEG‐PHB‐PEG aqueous solution and (d) its self‐assembled hydrogel with α‐CD. (Reprinted with permission from Ref . Copyright 2006). (e) Transmission electron microscopy image showing the self‐assembled micelles from PEG‐PHB‐PEG (5000‐3820‐5000) triblock copolymer. (Reprinted with permission from Ref . Copyright 2005 American Chemical Society). (f) In vitro release profiles for dextran‐FITC released from α‐CD–PEO–PHB–PEO hydrogels with various compositions. (Reprinted with permission from Ref . Copyright 2006 Elsevier)
[ Normal View | Magnified View ]
P3/4HB‐g‐bPEI grafted copolymer as nanovector for siRNA delivery. (a) Synthesis route of mP3/4HB‐acrylate and its mP3/4HB‐g‐bPEI grafted copolymer. (b) Confocal microscopy images showing the A549 cell uptake of (b) free siRNA and (c) P3/4HB‐g‐bPEI/siRNA complexes. (d) Lipofectamine and (e) bPEI were used as control. Cy3‐labeled siRNA appeared red fluorescence and the cell nuclei were stained blue by using 4',6‐diamidino‐2‐phenylindole (DAPI). (Reprinted with permission from Ref . Copyright 2012 Elsevier)
[ Normal View | Magnified View ]
PHOU‐g‐Jeffamine grafted copolymer and its thermo‐responsive behavior. (a) Synthetic procedure of PHOU‐g‐Jeffamine grafted copolymers through thiol‐ene click chemistry and (b) the temperature‐induced thermoresponsiveness of the copolymers in aqueous solution. (Reprinted with permission from Ref . Copyright 2012 Wiley‐VCH)
[ Normal View | Magnified View ]
Schematic diagram showing the synthesis of theranostic poly(3‐hydroxybutyrate) (PHB) nanoparticles using AGP synthase. The hybrid PHB–protein block copolymer can self‐assemble into a micellar structure with GFP and A33scFv displayed on the nanoparticle surface. Hydrophobic therapeutic molecules can be loaded into the core of the micelle for targeting delivery. (Reprinted with permission from Ref . Copyright 2014 Royal Society of Chemistry)
[ Normal View | Magnified View ]
Surface functionalization of hydrophobic poly(3‐hydroxybutyrate) (PHB) nanoparticles through polyhydroxyalkanoate (PHA) synthase‐catalyzed reaction for tumor‐specific drug delivery. (Reprinted with permission from Ref . Copyright 2011 Elsevier)
[ Normal View | Magnified View ]
Uptake of drug containing polyhydroxyalkanoate (PHA) nanoparticles by receptor‐mediated endocytosis. Drug containing PHA nanoparticles was coated with hypermannosylated rhAGP–PhaP or rhEGF–PhaP fusion protein to become drug delivery vectors. The two vectors were specifically bound to the surface of macrophages and hepatocellular carcinoma cells, respectively, and were taken up by both types of the cells via a receptor‐mediated endocytosis process. (Reprinted with permission from Ref . Copyright 2008 Elsevier)
[ Normal View | Magnified View ]
Schematic illustration of the biosynthesized polyhydroxyalkanoate (PHA) granule formation within the cytoplasm of microorganism which includes hydrophobic core of amorphous PHA surrounded by phospholipid and various proteins. (Reprinted with permission from Ref . Copyright 2013 Hindawi)
[ Normal View | Magnified View ]
Intracellular trafficking of using polyethylenimine (PEI)‐coated poly(3‐hydroxybutyrate‐co‐3‐hydroxyhexanoate) (PHBHHx) nanoparticles as delivery vehicles for cell targeting. MTOC, microtubule‐organizing center; ER, endoplasmic reticulum; eG, exocytic Golgi vesicle; eER, exocytic endoplasmic reticulum vesicle. (Reprinted with permission from Ref . Copyright 2014 Wiley‐VCH)
[ Normal View | Magnified View ]

Browse by Topic

Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement
Implantable Materials and Surgical Technologies > Nanomaterials and Implants

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts