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WIREs Nanomed Nanobiotechnol
Impact Factor: 9.182

Advances in single‐molecule fluorescent nanosensors

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Abstract Single‐molecule detection represents the ultimate sensitivity in measurement science with the characteristics of simplicity, rapidity, low sample consumption, and high signal‐to‐noise ratio and has attracted considerable attentions in biosensor development. In recent years, a variety of functional nanomaterials with unique chemical, optical, mechanical, and electronic features have been synthesized. The integration of single‐molecule detection with functional nanomaterials enables the construction of novel single‐molecule fluorescent nanosensors with excellent performance. Herein, we review the advance in single‐molecule fluorescent nanosensors constructed by novel nanomaterials including quantum dots, gold nanoparticles, upconversion nanoparticles, fluorescent conjugated polymer nanoparticles, nanosheets, and magnetic nanoparticles in the past decade (2011–2020), and discuss the strategies, features, and applications of single‐molecule fluorescent nanosensors in the detection of microRNAs, DNAs, enzymes, proteins, viruses, and live cells. Moreover, we highlight the future direction and challenges in this area. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vitro Nanoparticle‐Based Sensing Diagnostic Tools > Diagnostic Nanodevices
(a) QD‐based single‐molecule fluorescent nanosensor for Dam activity detection. Reprinted with permission from Ma, Liu, et al. (2017). Copyright 2017 The Royal Society of Chemistry. (b) QD‐based single‐molecule fluorescent nanosensor for M.SssI activity detection. Reprinted with permission from H. Zhang, Zhang, Yao, et al. (2019). Copyright 2019 American Chemical Society. (c) QD‐based single‐molecule fluorescent nanosensor for hOGG1 activity detection. Reprinted with permission from L.‐J. Wang et al. (2016). Copyright 2016 American Chemical Society. (d) QD‐based single‐molecule fluorescent nanosensor for TdT activity detection. Reprinted with permission from L.‐J. Wang, Luo, et al. (2017). Copyright 2017 The Royal Society of Chemistry. (e) QD‐base single‐molecule fluorescent nanosensor for ALP activity detection. Reprinted with permission from Ma, Liu, and Zhang (2019). Copyright 2019 The Royal Society of Chemistry. (f) AuNP‐based single‐molecule fluorescent nanosensor for PNK activity detection. Reprinted with permission from L.‐J. Wang, Zhang, et al. (2017). Copyright 2017 American Chemical Society
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(a) QD‐based single‐molecule fluorescent nanosensor for PKA activity detection. Reprinted with permission from L.‐J. Wang, Yang, and Zhang (2015). Copyright 2015 American Chemical Society. (b) QD‐based single‐molecule fluorescent nanosensor for OGT activity detection. Reprinted with permission from Hu et al. (2017). Copyright 2017 American Chemical Society
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(a) FCPNP‐based and AUNP‐based single‐molecule fluorescent nanosensor for GST activity detection. Reprinted with permission from Y. Han, Chen, et al. (2019). Copyright 2019 American Chemical Society. (b) FCPNP and MnO2 nanosheet‐based single‐molecule fluorescent nanosensor for AChE activity detection. Reprinted with permission from Y. Han, Ye, et al. (2019). Copyright 2019 The Royal Society of Chemistry
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(a) Fluorescent magnetic nanospheres‐based single‐molecule fluorescent nanosensor for multiple viruses assay. Reprinted with permission from Z. Wu et al. (2019). Copyright 2019 American Chemical Society. (b) Magnetic bead‐based single‐molecule fluorescent nanosensor for cancer cells detection. Reprinted with permission from Ma, Wei, and Zhang (2019). Copyright 2019 American Chemical Society
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(a) QD‐based single‐molecule fluorescent nanosensor for single DNA target detection. Reprinted with permission from (Z. Wang et al., 2012). Copyright 2012 The Royal Society of Chemistry. (b) QD‐based and MB‐based single‐molecule fluorescent nanosensor for multiplexed detection of two DNA targets. Reprinted with permission from Zhou et al. (2013). Copyright 2013 American Chemical Society. (c) FNP‐based and MB‐based single‐molecule fluorescent nanosensor for multiplexed detection of three DNA targets. Reprinted with permission from Pei et al. (2018). Copyright 2018 American Chemical Society. (d) QD‐based single‐molecule fluorescent nanosensor for DNA methylation detection. Reprinted with permission from Z.‐Y. Wang et al. (2018). Copyright 2018 The Royal Society of Chemistry
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(a) QD‐based single‐molecule fluorescent nanosensor for microRNA assay using two‐stage EXPAR. Reprinted with permission from Zhang and Zhang (2011). Copyright 2011 American Chemical Society. (b) QD‐based single‐molecule fluorescent nanosensor for microRNA detection using toehold‐mediated strand displacement cascade. Reprinted with permission from Ma, Zhang, and Zhang (2019). Copyright 2019 American Chemical Society. (c) AuNP‐based single‐molecule fluorescent nanosensor for microRNA detection using toehold‐mediated strand displacement cascade. Reprinted with permission from B. Li et al. (2020). Copyright 2020 American Chemical Society. (d) QD‐based single‐molecule fluorescent nanosensor for simultaneous measurement of multiple microRNAs. Reprinted with permission from Ma, Jiang, and Zhang (2020). Copyright 2020 Elsevier B. V.
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(a) UCNP and AUNP‐based single‐molecule fluorescent nanosensor for PSA detection. Reprinted with permission from X. Li et al. (2018). Copyright 2018 American Chemical Society. (b) UCNPs‐based single‐molecule fluorescent nanosensor for PSA detection. Reprinted with permission from Farka et al. (2017). Copyright 2017 American Chemical Society. (c) Magnetic bead‐based single‐molecule fluorescent nanosensor for cytokine detection. Reprinted with permission from W. Li et al. (2016). Copyright 2015 American Chemical Society
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Diagnostic Tools > Diagnostic Nanodevices
Diagnostic Tools > In Vitro Nanoparticle-Based Sensing
Diagnostic Tools > Biosensing

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