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WIREs Nanomed Nanobiotechnol

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Fluorine‐containing nanoemulsions for MRI cell tracking

Advanced Review

Jelena M. Janjic, Eric T. Ahrens

Published Online: Aug 20 2009

DOI: 10.1002/wnan.35

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Abstract In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for in vivo 19F MRI cell tracking. In this application, cells of interest are labeled in culture using a perfluorocarbon nanoemulsion. Labeled cells are introduced into a subject and tracked using 19F MRI or NMR spectroscopy. In the same imaging session, a high‐resolution, conventional (1H) image can be used to place the 19F‐labeled cells into anatomical context. Perfluorocarbon‐based 19F cell tracking is a useful technology because of the high specificity for labeled cells, ability to quantify cell accumulations, and biocompatibility. This technology can be widely applied to studies of inflammation, cellular regenerative medicine, and immunotherapy. Copyright © 2009 John Wiley & Sons, Inc. This article is categorized under: Diagnostic Tools > In Vivo Nanodiagnostics and Imaging

Cellular MRI using PFC nanoemulsion technology. PFC nanoemulsion is added to cultured cells that have been harvested from a subject or an engineered line. The labeled cells are transplanted into the subject and imaged using ¹⁹F and ¹H MRI in the same imaging session. The registered images are overlaid, yielding an image of the labeled cells in their anatomical context.

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Dual‐mode reagents for ¹⁹F MRI and fluorescent detection. Confocal microscopy of (a) labeled mouse DCs (b) and primary T cells labeled with FBPA (red). (Adapted from Ref 34).

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In vivo MRI of labeled T cells in the mouse model. The ¹⁹F image (pseudo‐color) shows a localized accumulation of T cells labeled with PFPE nanoemulsion in lymph nodes and the grayscale underlay is an anatomical ¹H image. Panels (a) and (b) display two consecutive 2 mm thick slices through the torso, and for anatomical orientation the kidneys (K) and gut (G) are noted. During imaging, the mouse was anesthetized with a ketamine/xylazine cocktail, connected to a mechanical ventilation apparatus, acquisitions were cardio‐respiratory gated, and body temperature was regulated at 37^°C. Data were collected for both ¹⁹F and ¹H in a single imaging session. (Adapted from Ref 34).

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In vivo MRI using PFC with dendritic cells (DCs) in mouse. Images of the labeled cells (i.e., ¹⁹F images) are displayed on a ‘hot‐iron’ intensity scale, and the anatomical (¹H) images are shown in grayscale. The three panels on the far left are a mouse quadriceps after intramuscular injection of DCs (asterisk indicates injection site). (a) ¹⁹F and ¹H images (from left to right) and a ‘composite’ ¹⁹F/¹H image. (b) The composite image of DC migration into the popliteal lymph node following a hind foot pad injection. (c) Composite image through the torso following intravenous inoculation with labeled DCs. Cells are apparent in the liver, spleen, and weakly in the lungs. (Data taken from Ref 19).

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DLS analysis of a linear PFPE nanoemulsion emulsified with F68 and PEI. Panel A displays the diameter distribution by light intensity, and B shows the diameter and PDI (error bars) followed over time at three different storage temperatures: 4 (▪), 25 (●), and 37 (▾ ^°C. (Adapted from Ref 34).

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Survey of PFCs for potential MRI applications.20, 22, 30–34.

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