This Title All WIREs
How to cite this WIREs title:
WIREs Nanomed Nanobiotechnol
Impact Factor: 7.689

Nanotubes in biosensing

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

Abstract Carbon nanotubes (CNTs) have extensively been used for electrochemical and optical biosensing due to the unique mechanical, chemical, and electrical properties. This review introduces two functionalization categories, noncovalent interaction along the CNTs sidewalls via physical adsorption or entrapment and covalent binding via carboxylate chemistry or nonselective attack of nanotube sidewalls by highly reactive species and gives an overview on the functionalized CNTs‐based biosensing methodologies for DNA, antigen‐antibody, cells, and other biological molecules. Furthermore, the in vivo near‐IR fluorescence biosensing application of CNTs with high photostability and efficiency is discussed. Finally, field‐effect transistors based on semiconductor CNTs are also summarized for ultrasensitive detection. Biosensors based on CNTs provide a significant avenue for the detection of biomolecules in vivo and in vitro applications. WIREs Nanomed Nanobiotechnol 2010 2 496–509 This article is categorized under: Diagnostic Tools > Biosensing

Covalent assembly of the single‐walled carbon nanotube (SWCNT)‐based GOD electrode. SWCNT was firstly immobilized via carbodiimide chemistry on the electrode surface, and then the amino derivative of the flavin adenine dinucleotide (FAD) cofactor was covalently attached to the carboxyl groups at the SWCNT tips. (Reprinted with permission from Ref 31. Copyright 2004 Wiley‐VCH Verlag GmbH & Co. KGaA).

[ Normal View | Magnified View ]

Noncovalent adsorption of single‐walled carbon nanotube with 1‐pyrenebutanoic acid, succinimidyl ester via π–π stacking. Amine groups on a biomolecule react with the anchored succinimidyl ester to form amide bonds for biomolecule immobilization. (Reprinted with permission from Ref 7. Copyright 2001 American Chemical Society).

[ Normal View | Magnified View ]

(a) Binding of thrombin on an single‐walled carbon nanotubes‐field‐effect transistor (SWCNT‐FET)‐based aptamer sensor. (b) The sensitivity of SWCNT‐FET aptamer sensor as a function of thrombin concentration. (c) The sensitivity of SWCNT‐FET aptamer sensor as a function of thrombin concentration. (Reprinted with permission from Ref 40. Copyright 2005 John Wiley & Sons, Inc.).

[ Normal View | Magnified View ]

Single‐molecule H2O2 detection: (a) Schematic of biotinylated DNA–single‐walled carbon nanotubes (SWCNT) binding to a glass surface with bovine serum albumin–biotin and Neutravidin. (b) Fitted traces from a movie showing single‐step SWCNT emission quenching upon perfusion of H2O2. (Reprinted with permission from Ref 35. Copyright 2009 Nature Publishing).

[ Normal View | Magnified View ]

(a) Preparation and enzyme‐catalyzed analysis of the designed cytosensor. (b) Differential pulse voltammetry (DPV) curves of HRP‐ConA/BGC/RGDS‐SWCNT/GCE obtained with BGC cell concentrations of 1 × 103, 5 × 103, 1 × 104, 5 × 104, 1 × 105, 5 × 105, 1 × 106, 6 × 106, and 1 × 107 cells mL−1 (from a′ to i′). Inset: plot of DPV peak current versus logarithm of BGC cell concentration. (Reprinted with permission from Ref 22. Copyright 2008 ACS).

[ Normal View | Magnified View ]

Schematic representation of (a) preparation procedure of glucose oxidase (GOD)–Au Nps/carbon nanotubes (CNTs)‐Ab2 tracer and (b) preparation of immunosensors and sandwich‐type electrochemical immunoassay. (Reprinted with permission from Ref 17. Copyright 2009 American Chemical Society).

[ Normal View | Magnified View ]

(a) Scheme for signaling biomolecular interaction by the assembly of single‐walled carbon nanotubes (SWCNT) and dye‐labeled single strand DNA. (b) Fluorescence emission spectra of 50 nM FAM‐labeled oligonucleotides (P1) in (a′) phosphate buffer (PBS), (b′)300 nM perfect cDNA (T1), (c′) SWCNT, and (d′) SWCNT + 300 nM T1. Inset: fluorescence intensity ratio of P1 and P1–SWCNT with F/F0 plotted against the logarithm of the concentration of T1. Excitation was at 480 nm, and emission was monitored at 528 nm. (Reprinted with permission from Ref 11. Copyright 2008 ACS).

[ Normal View | Magnified View ]

Related Articles

Catalyst‐functionalized nanomaterials
Nanoelectrodes for biological measurements

Browse by Topic

Diagnostic Tools > Biosensing

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts