This Title All WIREs
How to cite this WIREs title:
Impact Factor: 9.957

Processing of virus‐derived cytoplasmic primary‐microRNAs

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

MicoRNAs (miRNAs) are small noncoding RNAs that associate with Argonaute (AGO) family member proteins to mediate silencing of host mRNA transcripts. A number of DNA viruses and a single retrovirus exploit this pathway to generate their own miRNAs in an effort to self regulate or combat the host immune response to infection. While natural examples of viral miRNAs have been limited to nuclear viruses, manipulation of cytoplasmic‐restricted RNA virus genomes revealed the ability of these vectors to produce abundant levels of mature, functional miRNAs, exclusively within the cytoplasm. This novel processing mechanism is found to be dependent upon the ribonuclease (RNAse) III proteins Drosha and Dicer, while processing is able to proceed in the absence of the canonical dsRNA binding proteins DiGeorge syndrome critical region gene 8 (DGCR8), Tar RNA binding protein 2 (TRBP2), and protein kinase R activating protein (PACT). Processing of cytoplasmic restricted primary‐miRNA transcripts (c‐pri‐miRNAs) corresponds with virus‐induced redistribution of Drosha, implicating the formation of a noncanonical microprocessor. This review will discuss c‐pri‐miRNA processing, the mechanism of miRNA production, and the implications of a virus‐induced cytoplasmic microprocessor. WIREs RNA 2013, 4:463–471. doi: 10.1002/wrna.1169 This article is categorized under: RNA Processing > Processing of Small RNAs Regulatory RNAs/RNAi/Riboswitches > RNAi: Mechanisms of Action Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs

This WIREs title offers downloadable PowerPoint presentations of figures for non-profit, educational use, provided the content is not modified and full credit is given to the author and publication.

Download a PowerPoint presentation of all images

Small RNA pathways in insects and mammalians. In Drosophila melanogaster, virus‐derived dsRNA is cleaved by Dicer‐2 in to duplex RNAs, one strand of which mediates the targeting of pathogen RNA. In mammalians, miRNAs are produced in a stepwise fashion, via Drosha and Dicer, to produce a small RNA that mediates silencing of host transcripts.
[ Normal View | Magnified View ]
Proposed mechanism of c‐pri‐miRNA processing. (1) SINV infection leads to Drosha relocalization and the production of c‐pri‐miRNAs within the cytoplasm; (2) Drosha mediates the cytoplasmic cleavage of c‐pri‐miRNAs, releasing a pre‐miRNA that is (3) processed by Dicer. (4) One strand of the duplex is loaded into RISC where it (5) mediates post‐transcriptional silencing.
[ Normal View | Magnified View ]

Related Articles

Regulatory Non-Coding RNAs

Browse by Topic

Regulatory RNAs/RNAi/Riboswitches > RNAi: Mechanisms of Action
Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs
RNA Processing > Processing of Small RNAs

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts