This Title All WIREs
How to cite this WIREs title:
WIREs Syst Biol Med
Impact Factor: 4.192

Protein–membrane interactions: the virtue of minimal systems in systems biology

Full article on Wiley Online Library:   HTML PDF

Can't access this content? Tell your librarian.

Abstract The plasma membrane of cells can be viewed as a highly dynamic, regulated, heterogeneous environment with multiple functions. It constitutes the boundary of the cell, encapsulating all its components. Proteins interact with the membrane in many ways to accommodate essential processes, such as membrane trafficking, membrane protrusions, cytokinesis, signaling, and cell–cell communication. A vast amount of literature has already fostered our current understanding of membrane–protein interactions. However, many phenomena still remain to be understood, e.g., the exact mechanisms of how certain proteins cause or assist membrane transformations. Systems biology aims to predict biological processes on the basis of the set of molecules involved. Many key processes arise from interactions with the lipid membrane. Protein interactome maps do not consider such specific interactions, and thus cannot predict precise outcomes of the interactions of the involved proteins. These can only be inferred from experimental approaches. We describe examples of how an emergent behavior of protein–membrane interactions has been demonstrated by the use of minimal systems. These studies contribute to a deeper understanding of protein interactomes involving membranes and complement other approaches of systems biology. WIREs Syst Biol Med 2011 3 269–280 DOI: 10.1002/wsbm.119 This article is categorized under: Laboratory Methods and Technologies > Macromolecular Interactions, Methods

This WIREs title offers downloadable PowerPoint presentations of figures for non-profit, educational use, provided the content is not modified and full credit is given to the author and publication.

Download a PowerPoint presentation of all images

Schematics of different lipid systems.

[ Normal View | Magnified View ]

Interactions of network of proteins and lipids involved in clathrin‐mediated endocytosis (CME). Black circles refer to the components discussed in this review. In red are marked interactions of lipids; in green interactions of clathrin; in blue interactions of dynamin and in black interactions of AP2. (Adapted with permission from Ref 60. Copyright 2006 PLoS).

[ Normal View | Magnified View ]

Actin based motility. (a, b) Force generation by actin gel moving beads and microcapillary rods. (Reprinted with permission from Ref 42. Copyright 2010 Elsevier). (c) Schematic of actin arrangements in lamellipodia (solid box) and filopodia (dashed box). (d) The force generation mechanism by actin gel on a bead is similar to that at the lamellipodia (solid box in (c)). (e) Model based on actin branches facing forward with growth at the barbed ends. (f) A ‘primer’ based model based on new observations where an active zone pushes against the membrane. (g) Filopodia like protrusions reconstituted on giant unilamellar vesicles. (Reprinted with permission from Ref 43. Copyright 2008 Nature Publishing Group). Scale bar (a), (b), (g): 5 µm.

[ Normal View | Magnified View ]

Self organization by Min proteins. (a) Min waves on Escherichia coli polar‐extract‐supported lipid bilayers. (Reprinted with permission from Ref 35. Copyright 2008 AAAS). (b) Models of Min wave generation (Adapted with permission from Ref 36. Copyright 2007 John Wiley & Sons, Inc.). (c) Oscillations of MinD—GFP in vivo. (Reprinted with permission from Ref 29. Copyright 2001 Nature Publishing Group) Scale bar A: 50 µm C: 2 µm.

[ Normal View | Magnified View ]

Browse by Topic

Laboratory Methods and Technologies > Macromolecular Interactions, Methods

Access to this WIREs title is by subscription only.

Recommend to Your
Librarian Now!

The latest WIREs articles in your inbox

Sign Up for Article Alerts