Combining RNA‐binding domain(s) that are not found naturally together and fusing functional domains to RBDs can lead to engineered proteins with novel functions.

Phosphorylation of Pol II CTD determines which droplet Pol II should join and that may function as a switch for coupling transcription and splicing.

The network of RNA (red structures) and protein (circles) is determined by the dynamics of each interaction (grey lines).

Understanding how RNA molecules orchestrate gene expression pathways and malfunction in disease requires investigations of localization, interactions, and dynamics of RNA in living cells. In this review article, we highlight chemo‐enzymatic methods developed to make RNA accessible to analyses. In particular,chemo‐enzymatic strategies for RNA labeling, enrichment and detection of intrinsic modifications will be presented.